› 1rup

X-ray diffraction
1.4Å resolution

Crystal structure (G) of native cationic cyclization antibody 4C6 fab at pH 8.5 with a data set collected at APS beamline 19-ID

Released:
DOI: 10.2210/pdb1rup/pdb
PDB 1rup coloured by chain and viewed from the front.
PDB 1rup coloured by chain and viewed from the side.
PDB 1rup coloured by chain and viewed from the top.
Source organism: Mus musculus
Primary publication:
Probing the antibody-catalyzed water-oxidation pathway at atomic resolution.
Zhu X, Wentworth P, Wentworth AD, Eschenmoser A, Lerner RA, Wilson IA
Proc. Natl. Acad. Sci. U.S.A. 101 2247-52 (2004)
PMID: 14982995

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
immunoglobulin igg2a, heavy chain Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.22 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Ig gamma-2A chain C region, membrane-bound form Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01865 (Residues: 1-102; Coverage: 26%)
Gene name: Igh-1a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand BEZ 1 x BEZ
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 64.197Å b: 64.197Å c: 266.475Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.212

Quick links

Citations

3 review citations

Endogenous Generation of Singlet Oxygen and Ozone in Human and Animal Tissues: Mechanisms, Biological Significance, and Influence of Dietary Components.
Onyango AN. (2016)
2 more

1 mention without citation

Probing the antibody-catalyzed water-oxidation pathway at atomic resolution.
Zhu et al. (2004)