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CATABOLITE GENE ACTIVATOR PROTEIN (CAP) MUTANT/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

X-ray diffraction
2.7Å resolution
PDB entry 1ruo coloured by chain, front view.
PDB entry 1ruo coloured by chain, side view.
PDB entry 1ruo coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
hetero hexamer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA-binding transcriptional dual regulator CRP and DNA
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Multimeric state: hetero hexamer
Accessible surface area: 27248.38 Å2
Buried surface area: 10943.48 Å2
Dissociation area: 1,163.41 Å2
Dissociation energy (ΔGdiss): 12.84 kcal/mol
Dissociation entropy (TΔSdiss): 12.41 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-114543

Macromolecules

DNA-binding transcriptional dual regulator CRP
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 2-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Pfam:
InterPro:
CATH:
SCOP:
DNA-binding transcriptional dual regulator CRP in PDB entry 1ruo, assembly 1, front view.
DNA-binding transcriptional dual regulator CRP in PDB entry 1ruo, assembly 1, side view.
DNA-binding transcriptional dual regulator CRP in PDB entry 1ruo, assembly 1, top view.
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DNA chains C, E
Name: DNA (5'-D(*GP*CP*GP*AP*AP*AP*AP*AP*TP*GP*TP*GP*AP*T)-3')
Representative chains: C, E
Source organism: Escherichia coli [562]
Expression system: Not provided
Length: 14 nucleotides
Theoretical weight: 4.35 KDa
DNA (5'-D(*GP*CP*GP*AP*AP*AP*AP*AP*TP*GP*TP*GP*AP*T)-3') in PDB entry 1ruo, assembly 1, front view.
DNA (5'-D(*GP*CP*GP*AP*AP*AP*AP*AP*TP*GP*TP*GP*AP*T)-3') in PDB entry 1ruo, assembly 1, side view.
DNA (5'-D(*GP*CP*GP*AP*AP*AP*AP*AP*TP*GP*TP*GP*AP*T)-3') in PDB entry 1ruo, assembly 1, top view.
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DNA chains D, F
Name: DNA (5'-D(*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*TP*TP*TP*TP*CP*G )-3')
Representative chains: D, F
Source organism: Escherichia coli [562]
Expression system: Not provided
Length: 17 nucleotides
Theoretical weight: 5.15 KDa
DNA (5'-D(*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*TP*TP*TP*TP*CP*G )-3') in PDB entry 1ruo, assembly 1, front view.
DNA (5'-D(*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*TP*TP*TP*TP*CP*G )-3') in PDB entry 1ruo, assembly 1, side view.
DNA (5'-D(*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*TP*TP*TP*TP*CP*G )-3') in PDB entry 1ruo, assembly 1, top view.
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