› 1run

X-ray diffraction
2.7Å resolution

CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb1run/pdb
PDB 1run coloured by chain and viewed from the front.
PDB 1run coloured by chain and viewed from the side.
PDB 1run coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Aromatic hydrogen bond in sequence-specific protein DNA recognition.
Parkinson G, Gunasekera A, Vojtechovsky J, Zhang X, Kunkel TA, Berman H, Ebright RH
Nat. Struct. Biol. 3 837-41 (1996)
PMID: 8836098

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
cAMP-activated global transcriptional regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 2-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*GP*AP*AP*AP*AP*AP*TP*GP*TP*GP*AP*T)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 14 nucleotides
Theoretical weight: 4.35 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*TP*TP*TP*TP*CP*G )-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 17 nucleotides
Theoretical weight: 5.15 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CMP 2 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2221
Unit cell:
a: 136.93Å b: 152.8Å c: 76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Transcription activation by catabolite activator protein (CAP).
Busby et al. (1999)
1 more

17 mentions without citation

Conservation of the three-dimensional structure in non-homologous or unrelated proteins.
Sousounis et al. (2012)
16 more