› 1rrx

X-ray diffraction
2.1Å resolution

Crystallographic Evidence for Isomeric Chromophores in 3-Fluorotyrosyl-Green Fluorescent Protein

Released:
DOI: 10.2210/pdb1rrx/pdb
PDB entry 1rrx coloured by chain, front view.
PDB entry 1rrx coloured by chain, side view.
PDB entry 1rrx coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Crystallographic evidence for isomeric chromophores in 3-fluorotyrosyl-green fluorescent protein.
Bae JH, Paramita Pal P, Moroder L, Huber R, Budisa N
Chembiochem 5 720-2 (2004)
PMID: 15122645

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.85 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-229; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
2 modified residues:
Ligand YOF 9 x YOF
Ligand MFC 1 x MFC

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 51.003Å b: 62.43Å c: 70.931Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.272
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Fluorinated amino acids: compatibility with native protein structures and effects on protein-protein interactions.
Salwiczek et al. (2012)
6 other articles

1 mention without citation

Not all secretory granules are created equal: Partitioning of soluble content proteins.
Sobota et al. (2006)