1rr8

X-ray diffraction
2.6Å resolution

Structural Mechanisms of Camptothecin Resistance by Mutations in Human Topoisomerase I

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA topoisomerase 1 Chain: C
Molecule details ›
Chain: C
Length: 565 amino acids
Theoretical weight: 66.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P11387 (Residues: 203-765; Coverage: 74%)
Gene name: TOP1
Sequence domains:
Structure domains:
5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*T*GP*GP*AP*AP*AP*AP*AP*TP*TP*TP*TP*T)-3' Chain: A
Molecule details ›
Chain: A
Length: 22 nucleotides
Theoretical weight: 6.81 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*AP*AP*AP*AP*AP*TP*TP*TP*TP*TP*CP*CP*AP*AP*GP*TP*CP*TP*TP*TP*TP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.69 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 57.59Å b: 115.585Å c: 75.01Å
α: 90° β: 96.29° γ: 90°
R-values:
R R work R free
0.242 0.242 0.294
Expression systems:
  • Spodoptera frugiperda
  • Not provided