X-ray diffraction
2.6Å resolution

Crystal Structure of the Insulin Receptor Kinase in Complex with the SH2 Domain of APS


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Insulin receptor subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 35.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P06213 (Residues: 1005-1310; Coverage: 23%)
Gene name: INSR
Structure domains:
SH2B adapter protein 2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 114 amino acids
Theoretical weight: 12.89 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
  • Canonical: Q9Z200 (Residues: 401-510; Coverage: 18%)
Gene names: Aps, Sh2b2
Structure domains: SH2 domain
Molecule details ›
Chains: E, F
Length: 18 amino acids
Theoretical weight: 1.94 KDa

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 87.216Å b: 96.443Å c: 121.993Å
α: 90° β: 90° γ: 90°
R R work R free
0.229 0.229 0.277
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli