PDBe 1rqd

X-ray diffraction
3Å resolution

deoxyhypusine synthase holoenzyme in its low ionic strength, high pH crystal form with the inhibitor GC7 bound in the active site

Released:

Function and Biology Details

Reaction catalysed:
(1a) spermidine + NAD(+) = dehydrospermidine + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyhypusine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 41.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49366 (Residues: 1-369; Coverage: 100%)
Gene names: DHPS, DS
Sequence domains: Deoxyhypusine synthase
Structure domains: Deoxyhypusine synthase

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P3221
Unit cell:
a: 104.78Å b: 104.78Å c: 159.25Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.196 0.232
Expression system: Escherichia coli BL21(DE3)