› 1ro2

X-ray diffraction
1.6Å resolution

Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1- Triple mutant F50M/L107M/L110M manganese soak

Released:
DOI: 10.2210/pdb1ro2/pdb
PDB entry 1ro2 coloured by chain, front view.
PDB entry 1ro2 coloured by chain, side view.
PDB entry 1ro2 coloured by chain, top view.
Source organism: Sulfolobus islandicus
Primary publication:
Structure of a bifunctional DNA primase-polymerase.
Lipps G, Weinzierl AO, von Scheven G, Buchen C, Cramer P
Nat Struct Mol Biol 11 157-62 (2004)
PMID: 14730355

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SF3 helicase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 25.11 KDa
Source organism: Sulfolobus islandicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54324 (Residues: 40-249; Coverage: 23%)
Sequence domains: Bifunctional DNA primase/polymerase, N-terminal
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 45.811Å b: 119.907Å c: 41.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.252 0.264
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Phylogenomics of DNA topoisomerases: their origin and putative roles in the emergence of modern organisms.
Forterre et al. (2009)
9 more

2 mentions without citation

Structures to complement the archaeo-eukaryotic primases catalytic cycle description: What's next?
Boudet et al. (2015)
1 more