› 1rnl

X-ray diffraction
2.4Å resolution

THE NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL FROM NARL

Released:
DOI: 10.2210/pdb1rnl/pdb
PDB 1rnl coloured by chain and viewed from the front.
PDB 1rnl coloured by chain and viewed from the side.
PDB 1rnl coloured by chain and viewed from the top.
Source organism: Escherichia coli str. K-12 substr. W3110
Primary publication:
Structure of the Escherichia coli response regulator NarL.
Baikalov I, Schröder I, Kaczor-Grzeskowiak M, Grzeskowiak K, Gunsalus RP, Dickerson RE
Biochemistry 35 11053-61 (1996)
PMID: 8780507

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrate/nitrite response regulator protein NarL Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 23.83 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AF28 (Residues: 2-216; Coverage: 100%)
Gene names: JW1212, b1221, frdR, narL
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PT 6 x PT
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 61.037Å b: 78.321Å c: 115.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.251
Expression system: Escherichia coli

Quick links

Citations

30 review citations

To ∼P or Not to ∼P? Non-canonical activation by two-component response regulators.
Desai et al. (2017)
29 more

7 mentions without citation

Genetic evidence that transcription activation by RhaS involves specific amino acid contacts with sigma 70.
Bhende et al. (2000)
6 more