1rmq

X-ray diffraction
2Å resolution

Crystal structure of AphA class B acid phosphatase/phosphotransferase with osmiate mimicking the catalytic intermediate

Released:

Function and Biology Details

Reaction catalysed:
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Class B acid phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE22 (Residues: 26-237; Coverage: 100%)
Gene names: JW4015, aphA, b4055, napA, yjbP
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 91.732Å b: 66.447Å c: 91.523Å
α: 90° β: 121.3° γ: 90°
R-values:
R R work R free
0.187 0.183 0.23
Expression system: Escherichia coli