1rm1

X-ray diffraction
2.5Å resolution

Structure of a Yeast TFIIA/TBP/TATA-box DNA Complex

Released:
Source organism: Saccharomyces cerevisiae
Entry authors: Jin X, Gewirth DT, Geiger JH

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
TATA-box-binding protein Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 27.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P13393 (Residues: 1-240; Coverage: 100%)
Gene names: BTF1, SPT15, TBP1, YER148W
Structure domains: TATA-Binding Protein
Transcription initiation factor IIA subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 122 amino acids
Theoretical weight: 13.47 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32774 (Residues: 1-122; Coverage: 100%)
Gene names: TOA2, YKL058W
Structure domains:
Transcription initiation factor IIA large subunit Chain: C
Molecule details ›
Chain: C
Length: 286 amino acids
Theoretical weight: 32.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32773 (Residues: 1-286; Coverage: 100%)
Gene names: TOA1, YOR194C
Structure domains:
5'-D(*AP*TP*CP*GP*AP*TP*CP*GP*AP*TP*AP*TP*AP*AP*AP*AP*CP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.53 KDa
5'-D(P*CP*GP*TP*TP*TP*TP*AP*TP*AP*TP*CP*GP*AP*TP*CP*GP*AP*T)-3' Chain: E
Molecule details ›
Chain: E
Length: 18 nucleotides
Theoretical weight: 5.5 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: R32
Unit cell:
a: 173.25Å b: 173.25Å c: 164.07Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.186 0.252
Expression system: Escherichia coli