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X-ray diffraction
1.8Å resolution

The crystal structure of the D-aminoacylase mutant D366A

Released:

Function and Biology Details

Reaction catalysed:
N-acyl-D-amino acid + H(2)O = a carboxylate + D-amino acid
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-aminoacylase Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 53.42 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AGH8 (Residues: 1-484; Coverage: 100%)
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P212121
Unit cell:
a: 60.019Å b: 76.927Å c: 135.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.202
Expression system: Escherichia coli