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X-ray diffraction
2.6Å resolution

Structure of the complex between OspB-CT and bactericidal Fab-H6831

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Fab H6831 L-chain Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.22 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab H6831 H-chain Chain: B
Molecule details ›
Chain: B
Length: 221 amino acids
Theoretical weight: 23.66 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Outer surface protein B Chain: C
Molecule details ›
Chain: C
Length: 95 amino acids
Theoretical weight: 10.31 KDa
Source organism: Borreliella burgdorferi
Expression system: Escherichia coli
UniProt:
  • Canonical: P17739 (Residues: 202-296; Coverage: 34%)
Gene names: BB_A16, ospB
Structure domains: Outer Surface Protein A; domain 3
Outer surface protein B Chain: D
Molecule details ›
Chain: D
Length: 5 amino acids
Theoretical weight: 444 Da
Source organism: Borreliella burgdorferi
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 186.286Å b: 37.271Å c: 87.939Å
α: 90° β: 90.66° γ: 90°
R-values:
R R work R free
0.191 0.191 0.235
Expression system: Escherichia coli