› 1rj4

X-ray diffraction
2Å resolution

Structure of a Cell Wall Invertase Inhibitor from Tobacco in Complex with Cd2+

Released:
DOI: 10.2210/pdb1rj4/pdb
PDB 1rj4 coloured by chain and viewed from the front.
PDB 1rj4 coloured by chain and viewed from the side.
PDB 1rj4 coloured by chain and viewed from the top.
Source organism: Nicotiana tabacum
Primary publication:
The invertase inhibitor Nt-CIF from tobacco: a highly thermostable four-helix bundle with an unusual N-terminal extension.
Hothorn M, D'Angelo I, Márquez JA, Greiner S, Scheffzek K
J Mol Biol 335 987-95 (2004)
PMID: 14698293

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PMEI domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 151 amino acids
Theoretical weight: 16.09 KDa
Source organism: Nicotiana tabacum
Expression system: Escherichia coli
UniProt:
  • Canonical: O49908 (Residues: 20-166; Coverage: 100%)
Gene name: LOC107808322
Sequence domains: Plant invertase/pectin methylesterase inhibitor
Structure domains: Invertase/pectin methylesterase inhibitor family protein

Ligands and Environments

2 bound ligands:
Ligand CD 8 x CD
Ligand BTB 4 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 59.17Å b: 95.639Å c: 126.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.255
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Recent advances in plant cell wall proteomics.
Jamet et al. (2008)
1 more