1rhz

X-ray diffraction
3.5Å resolution

The structure of a protein conducting channel

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein translocase subunit SecY Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 47.48 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60175 (Residues: 1-436; Coverage: 100%)
Gene names: MJ0478, secY
Structure domains: SecY subunit domain
Protein translocase subunit SecE Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.45 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57817 (Residues: 1-74; Coverage: 100%)
Gene names: MJ0371, secE
Structure domains: Preprotein translocase SecE subunit
Preprotein translocase subunit SecG Chain: C
Molecule details ›
Chain: C
Length: 53 amino acids
Theoretical weight: 5.97 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: P60460 (Residues: 1-53; Coverage: 100%)
Gene names: MJ0912.1, secG

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 92.746Å b: 149.361Å c: 79.475Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.254 0.33
Expression system: Escherichia coli