1rg9

X-ray diffraction
2.5Å resolution

S-Adenosylmethionine synthetase complexed with SAM and PPNP

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 383 amino acids
Theoretical weight: 41.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A817 (Residues: 2-384; Coverage: 100%)
Gene names: JW2909, b2942, metK, metX
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 225.82Å b: 69.13Å c: 118.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.241
Expression system: Escherichia coli