X-ray diffraction
2.2Å resolution

Structural Studies of Streptococcus pneumoniae EPSP Synthase, Tetrahedral intermediate Bound State


Function and Biology Details

Reaction catalysed:
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
3-phosphoshikimate 1-carboxyvinyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 427 amino acids
Theoretical weight: 45.88 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
  • Canonical: Q9S400 (Residues: 1-427; Coverage: 100%)
Gene names: SP_1371, aroA
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 82.495Å b: 149.705Å c: 88.011Å
α: 90° β: 91.22° γ: 90°
R R work R free
0.243 0.196 0.236
Expression system: Escherichia coli