› 1rd5

X-ray diffraction
2.02Å resolution

Crystal structure of Tryptophan synthase alpha chain homolog BX1: a member of the chemical plant defense system

Released:
DOI: 10.2210/pdb1rd5/pdb
PDB 1rd5 coloured by chain and viewed from the front.
PDB 1rd5 coloured by chain and viewed from the side.
PDB 1rd5 coloured by chain and viewed from the top.
Source organism: Zea mays
Primary publication:
On the structural basis of the catalytic mechanism and the regulation of the alpha subunit of tryptophan synthase from Salmonella typhimurium and BX1 from maize, two evolutionarily related enzymes.
Kulik V, Hartmann E, Weyand M, Frey M, Gierl A, Niks D, Dunn MF, Schlichting I
J Mol Biol 352 608-20 (2005)
PMID: 16120446

Function and Biology Details

Reactions catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo octamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indole-3-glycerol phosphate lyase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.03 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: P42390 (Residues: 87-346; Coverage: 75%)
Gene name: BX1
Sequence domains: Tryptophan synthase alpha chain
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand MLA 2 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: F222
Unit cell:
a: 91.094Å b: 159.815Å c: 162.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.299
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Allosteric regulation of substrate channeling and catalysis in the tryptophan synthase bienzyme complex.
Dunn MF. (2012)
3 more

1 mention without citation

Functional classification of protein structures by local structure matching in graph representation.
Mills et al. (2018)