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ARF1[DELTA1-17]-GDP IN COMPLEX WITH A SEC7 DOMAIN CARRYING THE MUTATION OF THE CATALYTIC GLUTAMATE TO LYSINE

X-ray diffraction
1.46Å resolution
PDB entry 1r8s coloured by chain, front view.
PDB entry 1r8s coloured by chain, side view.
PDB entry 1r8s coloured by chain, top view.
Source organisms:
Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: ADP-ribosylation factor 1 and Cytohesin-2
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Multimeric state: hetero dimer
Accessible surface area: 16126.53 Å2
Buried surface area: 4884.85 Å2
Dissociation area: 1,668.48 Å2
Dissociation energy (ΔGdiss): 17.26 kcal/mol
Dissociation entropy (TΔSdiss): 12.25 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-160805
Assembly 2
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Assembly Name: ADP-ribosylation factor 1 and Cytohesin-2
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Multimeric state: hetero tetramer
Accessible surface area: 29640.16 Å2
Buried surface area: 12389.92 Å2
Dissociation area: 1,310.82 Å2
Dissociation energy (ΔGdiss): 2.59 kcal/mol
Dissociation entropy (TΔSdiss): 14.06 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-160806

Macromolecules

ADP-ribosylation factor 1
Chain: A
Length: 164 amino acids
Theoretical weight: 18.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84080 (Residues: 18-181; Coverage: 91%)
Gene name: ARF1
Pfam: ADP-ribosylation factor family
InterPro:
CATH: P-loop containing nucleotide triphosphate hydrolases
SCOP: G proteins
ADP-ribosylation factor 1 in PDB entry 1r8s, assembly 1, front view.
ADP-ribosylation factor 1 in PDB entry 1r8s, assembly 1, side view.
ADP-ribosylation factor 1 in PDB entry 1r8s, assembly 1, top view.
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Cytohesin-2
Chain: E
Length: 203 amino acids
Theoretical weight: 23.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99418 (Residues: 50-252; Coverage: 51%)
Gene names: ARNO, CYTH2, PSCD2, PSCD2L
Pfam: Sec7 domain
InterPro:
CATH:
SCOP: Sec7 domain
Cytohesin-2 in PDB entry 1r8s, assembly 1, front view.
Cytohesin-2 in PDB entry 1r8s, assembly 1, side view.
Cytohesin-2 in PDB entry 1r8s, assembly 1, top view.
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