1r6m

X-ray diffraction
2Å resolution

Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa In Complex With Phosphate

Released:
Source organism: Pseudomonas aeruginosa
Primary publication:
Probing the functional importance of the hexameric ring structure of RNase PH.
J. Biol. Chem. 279 755-64 (2004)
PMID: 14573594

Function and Biology Details

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease PH Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 25.68 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P50597 (Residues: 1-239; Coverage: 100%)
Gene names: PA5334, rph
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: R32
Unit cell:
a: 110.202Å b: 110.202Å c: 116.284Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.28
Expression system: Escherichia coli