X-ray diffraction
1.35Å resolution

Crystal Structure of DesIV double mutant (dTDP-glucose 4,6-dehydratase) from Streptomyces venezuelae with NAD and DAU bound


Function and Biology Details

Reaction catalysed:
dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
dTDP-glucose 4,6-dehydratase Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 36.5 KDa
Source organism: Streptomyces venezuelae
Expression system: Escherichia coli
  • Canonical: Q9ZGH3 (Residues: 1-337; Coverage: 100%)
Gene name: desIV
Sequence domains: GDP-mannose 4,6 dehydratase
Structure domains:

Ligands and Environments

Cofactor: Ligand NAD 1 x NAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 71.7Å b: 99.4Å c: 42.2Å
α: 90° β: 90° γ: 90°
R R work R free
0.176 0.174 0.216
Expression system: Escherichia coli