› 1r4y

Solution NMR

SOLUTION STRUCTURE OF THE DELETION MUTANT DELTA(7-22) OF THE CYTOTOXIC RIBONUCLEASE ALPHA-SARCIN

Released:
DOI: 10.2210/pdb1r4y/pdb
PDB entry 1r4y coloured by chain, ensemble of 25 models, front view.
PDB entry 1r4y coloured by chain, ensemble of 25 models, side view.
PDB entry 1r4y coloured by chain, ensemble of 25 models, top view.
Source organism: Aspergillus giganteus
Primary publication:
NMR structure of the noncytotoxic alpha-sarcin mutant Delta(7-22): the importance of the native conformation of peripheral loops for activity.
García-Mayoral MF, García-Ortega L, Lillo MP, Santoro J, Martínez del Pozo A, Gavilanes JG, Rico M, Bruix M
Protein Sci 13 1000-11 (2004)
PMID: 15044731

Function and Biology Details

Reaction catalysed: 
(1a) a 28S rRNA containing guanosine-adenosine pair = an (RNA fragment)-3'-adenosine-2',3'-cyclophosphate + a 5'-hydroxy-guanosine-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132816 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease alpha-sarcin Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 15.16 KDa
Source organism: Aspergillus giganteus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00655 (Residues: 28-177; Coverage: 91%)
Gene name: sar
Structure domains: Microbial ribonucleases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Fungal ribotoxins: molecular dissection of a family of natural killers.
Lacadena et al. (2007)
1 more

2 mentions without citation

NMR structure of the noncytotoxic alpha-sarcin mutant Delta(7-22): the importance of the native conformation of peripheral loops for activity.
García-Mayoral et al. (2004)
1 more