› 1r4f

X-ray diffraction
2.3Å resolution

Inosine-Adenosine-Guanosine Preferring Nucleoside Hydrolase From Trypanosoma vivax: Trp260Ala Mutant In Complex With 3-Deaza-Adenosine

Released:
DOI: 10.2210/pdb1r4f/pdb
PDB entry 1r4f coloured by chain, front view.
PDB entry 1r4f coloured by chain, side view.
PDB entry 1r4f coloured by chain, top view.
Source organism: Trypanosoma vivax
Primary publication:
Leaving group activation by aromatic stacking: an alternative to general acid catalysis.
Versées W, Loverix S, Vandemeulebroucke A, Geerlings P, Steyaert J
J Mol Biol 338 1-6 (2004)
PMID: 15050818

Function and Biology Details

Reaction catalysed: 
A purine nucleoside + H(2)O = D-ribose + a purine base
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine/uridine-preferring nucleoside hydrolase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 37.6 KDa
Source organism: Trypanosoma vivax
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GPQ4 (Residues: 2-327; Coverage: 100%)
Sequence domains: Inosine-uridine preferring nucleoside hydrolase
Structure domains: Ribonucleoside hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand AD3 1 x AD3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 51.996Å b: 74.865Å c: 81.369Å
α: 90° β: 102.291° γ: 90°
R-values:
R R work R free
0.208 0.205 0.257
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The place of metabolism in the origin of life.
Anet FA. (2004)
1 more

1 mention without citation

Gap junction intercellular communication and cytotoxicity in normal human cells after exposure to smoke condensates from cigarettes that burn or primarily heat tobacco.
McKarns et al. (2000)