› 1r16

X-ray diffraction
2Å resolution

Aplysia ADP ribosyl cyclase with bound pyridylcarbinol and R5P

Released:
DOI: 10.2210/pdb1r16/pdb
PDB 1r16 coloured by chain and viewed from the front.
PDB 1r16 coloured by chain and viewed from the side.
PDB 1r16 coloured by chain and viewed from the top.
Source organism: Aplysia californica
Primary publication:
ADP-ribosyl cyclase; crystal structures reveal a covalent intermediate.
Love ML, Szebenyi DM, Kriksunov IA, Thiel DJ, Munshi C, Graeff R, Lee HC, Hao Q
Structure 12 477-86 (2004)
PMID: 15016363

Function and Biology Details

Reactions catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.58 KDa
Source organism: Aplysia californica
Expression system: Escherichia coli
UniProt:
  • Canonical: P29241 (Residues: 25-282; Coverage: 100%)
Sequence domains: ADP-ribosyl cyclase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand N 2 x N
Ligand PYF 2 x PYF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 70.293Å b: 58.349Å c: 72.368Å
α: 90° β: 100.7° γ: 90°
R-values:
R R work R free
0.214 0.212 0.252
Expression system: Escherichia coli

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Citations

2 review citations

Macromolecular structures: Quality assessment and biological interpretation.
Salunke et al. (2017)
1 more