› 1qyq

X-ray diffraction
1.8Å resolution

Crystal Structure of the cyclized S65G Y66G GFP variant

Released:
DOI: 10.2210/pdb1qyq/pdb
PDB 1qyq coloured by chain and viewed from the front.
PDB 1qyq coloured by chain and viewed from the side.
PDB 1qyq coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.
Barondeau DP, Putnam CD, Kassmann CJ, Tainer JA, Getzoff ED
Proc. Natl. Acad. Sci. U.S.A. 100 12111-6 (2003)
PMID: 14523232

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.72 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 99%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR5 1 x CR5

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 50.937Å b: 62.858Å c: 71.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Structure-guided rational design of red fluorescent proteins: towards designer genetically-encoded fluorophores.
Eason et al. (2017)
17 more

2 mentions without citation

Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.
Barondeau et al. (2003)
1 more