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Crystal structure of matured green fluorescent protein R96A variant

X-ray diffraction
1.5Å resolution
PDB 1qyf coloured by chain and viewed from the front.
PDB 1qyf coloured by chain and viewed from the side.
PDB 1qyf coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Multimeric state: monomeric
Accessible surface area: 10400 Å2
Buried surface area: 300 Å2
Dissociation area: 100 Å2
Dissociation energy (ΔGdiss): 3 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Interface energy (ΔGint): -5 kcal/mol
Symmetry number: 1

Macromolecules

Green fluorescent protein
Chain: A
Length: 228 amino acids
Theoretical weight: 25.67 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-229; Coverage: 96%)
Gene name: GFP
Pfam: Green fluorescent protein
InterPro:
CATH: Green fluorescent protein
SCOP: Fluorescent proteins
PDB 1qyf contains 1 copy of Green fluorescent protein in assembly 1. This protein is highlighted and viewed from the front.
PDB 1qyf contains 1 copy of Green fluorescent protein in assembly 1. This protein is highlighted and viewed from the side.
PDB 1qyf contains 1 copy of Green fluorescent protein in assembly 1. This protein is highlighted and viewed from the top.
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