› 1qyf

X-ray diffraction
1.5Å resolution

Crystal structure of matured green fluorescent protein R96A variant

Released:
DOI: 10.2210/pdb1qyf/pdb
PDB 1qyf coloured by chain and viewed from the front.
PDB 1qyf coloured by chain and viewed from the side.
PDB 1qyf coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.
Barondeau DP, Putnam CD, Kassmann CJ, Tainer JA, Getzoff ED
Proc. Natl. Acad. Sci. U.S.A. 100 12111-6 (2003)
PMID: 14523232

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.67 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-229; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand EDO 1 x EDO
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 51.515Å b: 62.388Å c: 71.038Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.145 0.214
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Structure-guided rational design of red fluorescent proteins: towards designer genetically-encoded fluorophores.
Eason et al. (2017)
17 more

2 mentions without citation

Mechanism and energetics of green fluorescent protein chromophore synthesis revealed by trapped intermediate structures.
Barondeau et al. (2003)
1 more