› 1qy7

X-ray diffraction
2Å resolution

The structure of the PII protein from the cyanobacteria Synechococcus sp. PCC 7942

Released:
DOI: 10.2210/pdb1qy7/pdb
PDB entry 1qy7 coloured by chain, front view.
PDB entry 1qy7 coloured by chain, side view.
PDB entry 1qy7 coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
The structures of the PII proteins from the cyanobacteria Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803.
Xu Y, Carr PD, Clancy P, Garcia-Dominguez M, Forchhammer K, Florencio F, Vasudevan SG, Tandeau de Marsac N, Ollis DL
Acta Crystallogr D Biol Crystallogr 59 2183-90 (2003)
PMID: 14646076

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein P-II Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 112 amino acids
Theoretical weight: 12.39 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3F4 (Residues: 1-112; Coverage: 100%)
Gene names: Synpcc7942_0321, glnB
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand NI 3 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I4122
Unit cell:
a: 109.7Å b: 109.7Å c: 109.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.227
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Nitrogen regulation in bacteria and archaea.
Leigh et al. (2007)
5 more

10 mentions without citation

The PII-NAGK-PipX-NtcA Regulatory Axis of Cyanobacteria: A Tale of Changing Partners, Allosteric Effectors and Non-covalent Interactions.
Forcada-Nadal et al. (2018)
9 more