1qx7

X-ray diffraction
3.09Å resolution

Crystal structure of apoCaM bound to the gating domain of small conductance Ca2+-activated potassium channel

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
homo dimer
hetero dimer (preferred)
homo trimer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chains: A, B, I, M, R
Molecule details ›
Chains: A, B, I, M, R
Length: 148 amino acids
Theoretical weight: 17.14 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP29 (Residues: 2-149; Coverage: 99%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Small conductance calcium-activated potassium channel protein 2 Chain: D
Molecule details ›
Chain: D
Length: 85 amino acids
Theoretical weight: 10.22 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P70604 (Residues: 411-489; Coverage: 14%)
Gene name: Kcnn2
Sequence domains: Calmodulin binding domain

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P41212
Unit cell:
a: 79.93Å b: 79.93Å c: 220.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.286 0.286 0.308
Expression system: Escherichia coli BL21(DE3)