› 1qx4

X-ray diffraction
1.8Å resolution

Structrue of S127P mutant of cytochrome b5 reductase

Released:
DOI: 10.2210/pdb1qx4/pdb
PDB 1qx4 coloured by chain and viewed from the front.
PDB 1qx4 coloured by chain and viewed from the side.
PDB 1qx4 coloured by chain and viewed from the top.
Source organism: Rattus norvegicus
Primary publication:
The structure of the S127P mutant of cytochrome b5 reductase that causes methemoglobinemia shows the AMP moiety of the flavin occupying the substrate binding site.
Bewley MC, Davis CA, Marohnic CC, Taormina D, Barber MJ
Biochemistry 42 13145-51 (2003)
PMID: 14609324

Function and Biology Details

Reaction catalysed: 
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH-cytochrome b5 reductase 3 soluble form Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 31.33 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20070 (Residues: 34-301; Coverage: 89%)
Gene names: Cyb5r3, Dia1
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 77.236Å b: 48.004Å c: 77.247Å
α: 90° β: 107.69° γ: 90°
R-values:
R R work R free
0.207 0.207 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Human cytochrome b5 reductase: structure, function, and potential applications.
Elahian et al. (2014)
1 more