X-ray diffraction
1.8Å resolution

Structrue of S127P mutant of cytochrome b5 reductase


Function and Biology Details

Reaction catalysed:
NADH + 2 ferricytochrome b5 = NAD(+) + H(+) + 2 ferrocytochrome b5
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
NADH-cytochrome b5 reductase 3 soluble form Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 31.33 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
  • Canonical: P20070 (Residues: 34-301; Coverage: 89%)
Gene names: Cyb5r3, Dia1
Structure domains:

Ligands and Environments

Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P21
Unit cell:
a: 77.236Å b: 48.004Å c: 77.247Å
α: 90° β: 107.69° γ: 90°
R R work R free
0.207 0.207 0.241
Expression system: Escherichia coli