› 1qs0

X-ray diffraction
2.4Å resolution

Crystal Structure of Pseudomonas Putida 2-oxoisovalerate Dehydrogenase (Branched-Chain Alpha-Keto Acid Dehydrogenase, E1B)

Released:
DOI: 10.2210/pdb1qs0/pdb
PDB entry 1qs0 coloured by chain, front view.
PDB entry 1qs0 coloured by chain, side view.
PDB entry 1qs0 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal structure of 2-oxoisovalerate and dehydrogenase and the architecture of 2-oxo acid dehydrogenase multienzyme complexes.
Aevarsson A, Seger K, Turley S, Sokatch JR, Hol WG
Nat Struct Biol 6 785-92 (1999)
PMID: 10426958

Function and Biology Details

Reaction catalysed: 
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140547 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2-oxoisovalerate dehydrogenase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 407 amino acids
Theoretical weight: 45.59 KDa
Source organism: Pseudomonas putida
Expression system: Pseudomonas putida
UniProt:
  • Canonical: P09060 (Residues: 2-408; Coverage: 99%)
Gene name: bkdA1
Sequence domains:
Structure domains: Rossmann fold
2-oxoisovalerate dehydrogenase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 338 amino acids
Theoretical weight: 37.51 KDa
Source organism: Pseudomonas putida
Expression system: Pseudomonas putida
UniProt:
  • Canonical: P09061 (Residues: 2-339; Coverage: 100%)
Gene name: bkdA2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 1 x TPP
2 bound ligands:
Ligand MG 1 x MG
Ligand COI 1 x COI
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I4122
Unit cell:
a: 101.34Å b: 101.34Å c: 381.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.265
Expression system: Pseudomonas putida

Quick links

Citations

10 review citations

Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions.
Perham RN. (2000)
9 more

5 mentions without citation

The remarkable structural and functional organization of the eukaryotic pyruvate dehydrogenase complexes.
Zhou et al. (2001)
4 more