› 1qqq

X-ray diffraction
1.5Å resolution

CRYSTAL STRUCTURE ANALYSIS OF SER254 MUTANT OF ESCHERICHIA COLI THYMIDYLATE SYNTHASE

Released:
DOI: 10.2210/pdb1qqq/pdb
PDB entry 1qqq coloured by chain, front view.
PDB entry 1qqq coloured by chain, side view.
PDB entry 1qqq coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Drug-resistant variants of Escherichia coli thymidylate synthase: effects of substitutions at Pro-254.
Fantz C, Shaw D, Jennings W, Forsthoefel A, Kitchens M, Phan J, Minor W, Lebioda L, Berger FG, Spencer HT
Mol Pharmacol 57 359-66 (2000)
PMID: 10648646

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-141680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 30.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A884 (Residues: 1-264; Coverage: 100%)
Gene names: JW2795, b2827, thyA
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
2 modified residues:
Ligand CXM 1 x CXM
Ligand CME 4 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I213
Unit cell:
a: 132.437Å b: 132.437Å c: 132.437Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.238
Expression system: Escherichia coli

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Citations

1 review citation

Myeloprotection with drug-resistance genes.
Banerjee et al. (2002)
4 other articles

3 mentions without citation

Acetylation of the transcriptional repressor Ume6p allows efficient promoter release and timely induction of the meiotic transient transcription program in yeast.
Law et al. (2014)
2 more