1qo0

X-ray diffraction
2.25Å resolution

Amide receptor of the amidase operon of Pseudomonas aeruginosa (AmiC) complexed with the negative regulator AmiR.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aliphatic amidase expression-regulating protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 385 amino acids
Theoretical weight: 42.96 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Pseudomonas aeruginosa
UniProt:
  • Canonical: P27017 (Residues: 1-385; Coverage: 100%)
Gene names: PA3364, amiC
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold
Aliphatic amidase regulator Chains: D, E
Molecule details ›
Chains: D, E
Length: 196 amino acids
Theoretical weight: 21.9 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Pseudomonas aeruginosa
UniProt:
  • Canonical: P10932 (Residues: 1-196; Coverage: 100%)
Gene names: PA3363, amiR
Sequence domains: ANTAR domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: C2
Unit cell:
a: 308.44Å b: 67.15Å c: 76.41Å
α: 90° β: 103.33° γ: 90°
R-values:
R R work R free
0.186 0.186 0.256
Expression system: Pseudomonas aeruginosa