1qn5

X-ray diffraction
1.93Å resolution

Crystal structure of the G(-26) Adenovirus major late promoter TATA box variant bound to wild-type TBP (Arabidopsis thaliana TBP isoform 2). TATA element recognition by the TATA box-binding protein has been conserved throughout evolution.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TATA-box-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 22.4 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P28147 (Residues: 1-200; Coverage: 100%)
Gene names: At3g13445, MRP15.10, TBP1
Structure domains: TATA-Binding Protein
DNA (5'-D(*GP*CP*TP*AP*TP*AP*AP*GP*AP*GP*GP*GP*CP*A)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 14 nucleotides
Theoretical weight: 4.35 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
DNA (5'-D(*TP*GP*CP*CP*CP*TP*CP*TP*TP*AP*TP*AP*GP*C)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 14 nucleotides
Theoretical weight: 4.21 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 41.8Å b: 146.7Å c: 57.4Å
α: 90° β: 90.5° γ: 90°
R-values:
R R work R free
0.196 0.196 0.252
Expression systems:
  • Escherichia coli
  • Not provided