› 1qm5

X-ray diffraction
2Å resolution

Phosphorylase recognition and phosphorylysis of its oligosaccharide substrate: answers to a long outstanding question

Released:
DOI: 10.2210/pdb1qm5/pdb
PDB entry 1qm5 coloured by chain, front view.
PDB entry 1qm5 coloured by chain, side view.
PDB entry 1qm5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Phosphorylase recognition and phosphorolysis of its oligosaccharide substrate: answers to a long outstanding question.
Watson KA, McCleverty C, Geremia S, Cottaz S, Driguez H, Johnson LN
EMBO J 18 4619-32 (1999)
PMID: 10469642

Function and Biology Details

Reaction catalysed: 
((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltodextrin phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 796 amino acids
Theoretical weight: 90.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00490 (Residues: 2-797; Coverage: 100%)
Gene names: JW5689, b3417, malP
Sequence domains: Carbohydrate phosphorylase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
Carbohydrate polymer : NEW Components: GLC, SGC
Carbohydrate polymer
2 bound ligands:
Ligand PO4 2 x PO4
Ligand GLC 2 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 107.41Å b: 61.5Å c: 132.38Å
α: 90° β: 105.03° γ: 90°
R-values:
R R work R free
0.228 0.228 0.261
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
12 more

1 mention without citation

Crystal structure of glycogen synthase: homologous enzymes catalyze glycogen synthesis and degradation.
Buschiazzo et al. (2004)