1qln

X-ray diffraction
2.4Å resolution

STRUCTURE OF A TRANSCRIBING T7 RNA POLYMERASE INITIATION COMPLEX

Released:
Source organism: Escherichia phage T7
Primary publication:
Structure of a transcribing T7 RNA polymerase initiation complex.
Science 286 2305-9 (1999)
PMID: 10600732

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Structure domains:
DNA (5-D(P*TP*AP*AP*TP*AP*CP*GP*AP*CP*TP*CP*AP*CP*TP*A)-3) Chain: N
Molecule details ›
Chain: N
Length: 17 nucleotides
Theoretical weight: 5.15 KDa
Source organism: Enterobacteria phage T7
Expression system: Not provided
DNA (5- D (P*CP*TP*CP*CP*CP*TP*AP*TP*AP*GP*TP*GP*AP*GP*TP*CP*GP*TP* AP*TP*TP*A)-3) Chain: T
Molecule details ›
Chain: T
Length: 22 nucleotides
Theoretical weight: 6.72 KDa
Source organism: Enterobacteria phage T7
Expression system: Not provided
RNA (5- R(PPP*GP*GP*G)-3) Chain: R
Molecule details ›
Chain: R
Length: 3 nucleotides
Theoretical weight: 1.15 KDa
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 221.2Å b: 73.6Å c: 80.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.267
Expression systems:
  • Escherichia coli BL21
  • Not provided