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X-ray diffraction
2.3Å resolution

T4 Phage B-Glucosyltransferase, Substrate Binding and Proposed Catalytic Mechanism

Released:

Function and Biology Details

Reaction catalysed:
Transfers a beta-D-glucosyl residue from UDP-alpha-D-glucose to a hydroxymethylcytosine residue in DNA
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA beta-glucosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.72 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P04547 (Residues: 1-351; Coverage: 100%)
Gene name: bgt
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: P212121
Unit cell:
a: 69.46Å b: 102.47Å c: 59.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.29
Expression system: Escherichia coli