1qk5

X-ray diffraction
1.6Å resolution

TOXOPLASMA GONDII HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE WITH XMP, PYROPHOSPHATE AND TWO MG2+ IONS

Released:

Function and Biology Details

Reactions catalysed:
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
XMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine-xanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.66 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q26997 (Residues: 1-230; Coverage: 100%)
Gene name: HPRT
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 55.208Å b: 112.255Å c: 144.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.261
Expression system: Escherichia coli BL21(DE3)