1qjg

X-ray diffraction
2.3Å resolution

Crystal structure of delta5-3-ketosteroid isomerase from Pseudomonas testosteroni in complex with equilenin

Released:

Function and Biology Details

Reaction catalysed:
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 125 amino acids
Theoretical weight: 13.42 KDa
Source organism: Comamonas testosteroni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00947 (Residues: 1-125; Coverage: 100%)
Gene name: ksi
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 73.9Å b: 72.5Å c: 80.8Å
α: 90° β: 104.3° γ: 90°
R-values:
R R work R free
0.205 0.205 0.271
Expression system: Escherichia coli BL21(DE3)