1qj5

X-ray diffraction
1.8Å resolution

Crystal structure of 7,8-diaminopelargonic acid synthase

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 47.31 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12995 (Residues: 1-429; Coverage: 100%)
Gene names: JW0757, b0774, bioA
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 58.338Å b: 55.556Å c: 120.884Å
α: 90° β: 96.97° γ: 90°
R-values:
R R work R free
0.175 0.175 0.226
Expression system: Escherichia coli BL21(DE3)