› 1qbj

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE ZALPHA Z-DNA COMPLEX

Released:
DOI: 10.2210/pdb1qbj/pdb
PDB 1qbj coloured by chain and viewed from the front.
PDB 1qbj coloured by chain and viewed from the side.
PDB 1qbj coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the Zalpha domain of the human editing enzyme ADAR1 bound to left-handed Z-DNA.
Schwartz T, Rould MA, Lowenhaupt K, Herbert A, Rich A
Science 284 1841-5 (1999)
PMID: 10364558

Function and Biology Details

Reaction catalysed: 
Adenine in double-stranded RNA + H(2)O = hypoxanthine in double-stranded RNA + NH(3)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Double-stranded RNA-specific adenosine deaminase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 81 amino acids
Theoretical weight: 9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55265 (Residues: 127-209; Coverage: 7%)
Gene names: ADAR, ADAR1, DSRAD, G1P1, IFI4
Sequence domains: Adenosine deaminase z-alpha domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*TP*CP*GP*CP*GP*CP*G)-3') Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 7 nucleotides
Theoretical weight: 2.11 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4212
Unit cell:
a: 85.9Å b: 85.9Å c: 71.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

54 review citations

High-throughput techniques enable advances in the roles of DNA and RNA secondary structures in transcriptional and post-transcriptional gene regulation.
Georgakopoulos-Soares et al. (2022)
53 more

23 mentions without citation

Thermodynamic Model for B-Z Transition of DNA Induced by Z-DNA Binding Proteins.
Lee et al. (2018)
22 more