› 1qb8

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURES OF ADENINE PHOSPHORIBOSYLTRANSFERASE FROM LEISHMANIA DONOVANI

Released:
DOI: 10.2210/pdb1qb8/pdb
PDB entry 1qb8 coloured by chain, front view.
PDB entry 1qb8 coloured by chain, side view.
PDB entry 1qb8 coloured by chain, top view.
Source organism: Leishmania donovani
Primary publication:
Crystal structures of adenine phosphoribosyltransferase from Leishmania donovani.
Phillips CL, Ullman B, Brennan RG, Hill CP
EMBO J 18 3533-45 (1999)
PMID: 10393170

Function and Biology Details

Reaction catalysed: 
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173273 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
adenine phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.1 KDa
Source organism: Leishmania donovani
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27679 (Residues: 2-237; Coverage: 100%)
Gene name: LDHU3_26.0190
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand AMP 1 x AMP
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P6122
Unit cell:
a: 64.04Å b: 64.04Å c: 235.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.263
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Structural analyses reveal two distinct families of nucleoside phosphorylases.
Pugmire et al. (2002)
4 more

4 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
3 more