› 1qae

X-ray diffraction
2.05Å resolution

THE ACTIVE SITE OF SERRATIA ENDONUCLEASE CONTAINS A CONSERVED MAGNESIUM-WATER CLUSTER

Released:
DOI: 10.2210/pdb1qae/pdb
PDB entry 1qae coloured by chain, front view.
PDB entry 1qae coloured by chain, side view.
PDB entry 1qae coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
The active site of Serratia endonuclease contains a conserved magnesium-water cluster.
Miller MD, Cai J, Krause KL
J Mol Biol 288 975-87 (1999)
PMID: 10329193

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 26.74 KDa
Source organism: Serratia marcescens
Expression system: Serratia marcescens
UniProt:
  • Canonical: P13717 (Residues: 22-266; Coverage: 100%)
Gene names: nuc, nucA
Sequence domains: DNA/RNA non-specific endonuclease
Structure domains: Extracellular Endonuclease, subunit A

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 106.7Å b: 74.5Å c: 68.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.167 0.21
Expression system: Serratia marcescens

Quick links

Citations

7 review citations

Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.
Chevalier et al. (2001)
6 more

3 mentions without citation

Note: On the universality of proximal radial distribution functions of proteins.
Lin et al. (2011)
2 more