1q9m

X-ray diffraction
2.3Å resolution

Three dimensional structures of PDE4D in complex with roliprams and implication on inhibitor selectivity

Released:

Function and Biology Details

Reaction catalysed:
Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-specific 3',5'-cyclic phosphodiesterase 4D Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 360 amino acids
Theoretical weight: 41.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q08499 (Residues: 381-740; Coverage: 45%)
Gene names: DPDE3, PDE4D
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 99.272Å b: 112.493Å c: 160.908Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.259
Expression system: Escherichia coli BL21