Structure analysis

Crystal Structure of CCdAP-Puromycin bound at the Peptidyl transferase center of the 50S ribosomal subunit

X-ray diffraction
3.2Å resolution
Source organism: Haloarcula marismortui
Assembly composition:
hetero 31-mer (preferred)
Entry contents: 28 distinct polypeptide molecules
2 distinct RNA molecules
1 distinct DNA/RNA hybrid molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 31-mer
Accessible surface area: 436689.61 Å2
Buried surface area: 207675.4 Å2
Dissociation area: 466.4 Å2
Dissociation energy (ΔGdiss): 1.19 kcal/mol
Dissociation entropy (TΔSdiss): 9.66 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-113089

Macromolecules

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Chain: O
Length: 186 amino acids
Theoretical weight: 20.51 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P14123 (Residues: 2-187; Coverage: 100%)
Gene names: rpl18, rrnAC1593
Pfam: Ribosomal large subunit proteins 60S L5, and 50S L18
InterPro: Large ribosomal subunit protein uL18 eukaryotic/archaeal
CATH: Nucleotidyltransferase; domain 5
SCOP: Ribosomal protein L18 and S11

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Chain: W
Length: 70 amino acids
Theoretical weight: 7.76 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P10971 (Residues: 2-71; Coverage: 99%)
Gene names: rpl29, rrnAC1604
Pfam: Ribosomal L29 protein
InterPro:
CATH: Helix Hairpins
SCOP: Ribosomal protein L29 (L29p)

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Chain: Y
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P18138 (Residues: 2-92; Coverage: 99%)
Gene names: rpl31e, rrnAC3113
Pfam: Ribosomal protein L31e
InterPro:
CATH: Ribosomal Protein L31e; Chain: W;
SCOP: Ribosomal protein L31e

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Chain: 1
Length: 73 amino acids
Theoretical weight: 8.1 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P60619 (Residues: 11-82; Coverage: 78%)
Gene names: rpl37ae, rrnAC1669
Pfam: Ribosomal L37ae protein family
InterPro:
CATH: N-terminal domain of TfIIb
SCOP: Ribosomal protein L37ae

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Chain: 3
Length: 48 amino acids
Theoretical weight: 5.84 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P22452 (Residues: 2-50; Coverage: 96%)
Gene names: rpl39e, rrnAC3112
Pfam: Ribosomal L39 protein
InterPro:
CATH: Ribosomal protein L39e
SCOP: Ribosomal protein L39e

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Chain: 4
Length: 92 amino acids
Theoretical weight: 10.82 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P32411 (Residues: 1-92; Coverage: 100%)
Gene names: rpl44e, rrnAC3514
Pfam: Ribosomal protein L44
InterPro:
CATH: Nuclear Transport Factor 2; Chain: A,
SCOP: Ribosomal protein L44e

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Name: 23S ribosomal RNA
Representative chains: A
Length: 2922 nucleotides
Theoretical weight: 946.03 KDa
Rfam: Archaeal large subunit ribosomal RNA

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Name: 5S ribosomal RNA
Representative chains: B
Length: 122 nucleotides
Theoretical weight: 39.32 KDa
Rfam: 5S ribosomal RNA

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Name: CCdA-P-Puromycin
Representative chains: 5
Length: 3 nucleotides
Theoretical weight: 879 Da

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