1q74

X-ray diffraction
1.7Å resolution

The Crystal Structure of 1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside Deacetylase (MshB)

Released:

Function and Biology Details

Reaction catalysed:
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H(2)O = 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + acetate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 303 amino acids
Theoretical weight: 31.78 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WJN3 (Residues: 1-303; Coverage: 100%)
Gene names: Rv1170, mshB
Sequence domains: GlcNAc-PI de-N-acetylase
Structure domains: LmbE-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 56.794Å b: 73.964Å c: 85.587Å
α: 102.09° β: 108.16° γ: 97.18°
R-values:
R R work R free
0.196 0.194 0.228
Expression system: Escherichia coli BL21(DE3)