› 1q5j

X-ray diffraction
2.1Å resolution

Crystal structure of bacteriorhodopsin mutant P91A crystallized from bicelles

Released:
DOI: 10.2210/pdb1q5j/pdb
PDB entry 1q5j coloured by chain, front view.
PDB entry 1q5j coloured by chain, side view.
PDB entry 1q5j coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptors.
Yohannan S, Faham S, Yang D, Whitelegge JP, Bowie JU
Proc Natl Acad Sci U S A 101 959-63 (2004)
PMID: 14732697

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 26.9 KDa
Source organism: Halobacterium salinarum
Expression system: Escherichia coli
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
Ligand RET 2 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 44.527Å b: 108.3Å c: 55.818Å
α: 90° β: 113.41° γ: 90°
R-values:
R R work R free
0.218 0.216 0.263
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Folding of helical membrane proteins: the role of polar, GxxxG-like and proline motifs.
Senes et al. (2004)
27 more

6 mentions without citation

The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptors.
Yohannan et al. (2004)
5 more