X-ray diffraction
2.3Å resolution

Crystal structure of bacteriorhodopsin mutant P186A crystallized from bicelles


Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 26.9 KDa
Source organism: Halobacterium salinarum
Expression system: Escherichia coli
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 44.629Å b: 108.721Å c: 56.093Å
α: 90° β: 113.46° γ: 90°
R R work R free
0.198 0.194 0.256
Expression system: Escherichia coli