X-ray diffraction
1.58Å resolution

S65T Q80R T203C Green Fluorescent Protein (GFP) pH 5.5

Source organism: Aequorea victoria
Primary publication:
Local complexity of amino acid interactions in a protein core.
Proc Natl Acad Sci U S A 101 111-6 (2004)
PMID: 14684834

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.95 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 50.969Å b: 62.333Å c: 68.554Å
α: 90° β: 90° γ: 90°
R R work R free
0.208 0.208 0.233
Expression system: Escherichia coli BL21(DE3)