1q4a

X-ray diffraction
1.45Å resolution

S65T Q80R Green Fluorescent Protein (GFP) pH 8.5

Released:
Source organism: Aequorea victoria
Primary publication:
Local complexity of amino acid interactions in a protein core.
Proc. Natl. Acad. Sci. U.S.A. 101 111-6 (2004)
PMID: 14684834

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.95 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 50.864Å b: 62.351Å c: 69.174Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.201
Expression system: Escherichia coli BL21(DE3)